Handling blood, plasma, and serum: films, clots, and unreliable surfaces
Biological fluids clot, foam from protein, and coat the tip in a film that fools level detection. What a class for whole blood or serum has to account for.
Liquid handling in context: assays, sample prep, and workflows where getting the volume right decides the result.
Biological fluids clot, foam from protein, and coat the tip in a film that fools level detection. What a class for whole blood or serum has to account for.
Mass spec quantitation is only as good as the standards and dilutions feeding it. Here is how to keep low-volume transfers accurate for LC-MS/MS.
ELISA lives or dies on even, gentle, well-timed liquid handling. Here are the dispense, wash, and reagent-addition settings that keep plates consistent.
Setting up PCR plates by hand is tedious and error prone. The liquid-class settings that protect ratio fidelity at a few microliters and keep amplicon out.
Normalization takes a plate of unequal concentrations to a common target. Variable volumes, one class, and a minimum-transfer floor that decides feasibility.
DMSO is the backbone solvent of compound libraries and one of the hardest liquids to pipette well. Viscosity, water uptake, and freeze-thaw all move the target.
SPRI and magnetic-bead cleanups live or die on supernatant removal. The class settings that clear liquid off an engaged pellet without carrying beads away.
Library prep stacks dozens of small, ratio-sensitive transfers. Here is how to automate it without letting pipetting error compound into failed libraries.